Reporter

Part:BBa_K1122011

Designed by: Lina Gasiūnaitė   Group: iGEM13_Edinburgh   (2013-08-13)

Genabler acceptor lacZα cassette

A Genabler Acceptor Vector was engineered for joining assembled parts. To be compatible with Genabler assembly, lacZ'α (BBa_J33207) acceptor cassette is flanked by two outward-facing EarI sites. When using Genabler assembly, blue visual marker enables easy selection of the colonies containing a re-ligated pAcceptor2 plasmid, therefore, less screening for successful assemblies is needed.

Acceptor2.jpg

Figure 1. Home-made DH5α competent E. coli cells, transformed with Genabler Acceptor Vector, express lacZ'α. Due to this colonies are blue on LB plates supplied with X-gal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5


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